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axiovert 200 inverted microscope with hoffman modulation contrast optics  (Carl Zeiss)


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    Structured Review

    Carl Zeiss axiovert 200 inverted microscope with hoffman modulation contrast optics
    Axiovert 200 Inverted Microscope With Hoffman Modulation Contrast Optics, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hoffman+modulation+contrast+microscope/axioskop%E2%80%99+microscope+zeiss/pm39874642-112-20-24
    Average 90 stars, based on 1 article reviews
    axiovert 200 inverted microscope with hoffman modulation contrast optics - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Developmental toxicity assessment of common excipients using a stem cell-based in vitro morphogenesis model
    Article Snippet: .. Measurement of morphometric parameters EBs were removed from hanging drops and grouped together for photography using an AxioCam MRm digital camera connected to an Axiovert 200 microscope with Hoffman modulation-contrast optics (Carl Zeiss, Thornwood, NY). ..

    Article Title: Embryoid body test with morphological and molecular endpoints implicates potential developmental toxicity of trans -resveratrol
    Article Snippet: .. Morphometric analyses EBs were placed together in a dish filled with phosphate-buffered saline for photography, using an AxioCam MRm digital camera connected to an Axiovert 200 microscope with Hoffman modulation-contrast optics (Carl Zeiss, Thornwood, NY). ..

    other:

    Article Title: Comparative Analysis of Number and Competence in Meiotic Maturation of Preovulatory Oocytes of C57Bl/6J Mice and Their F1 Hybrids after Stimulation with Gonadotropin.
    Article Snippet: The study examined maturation of preovulatory germinal vesicles oocytes (GV oocytes) induced by gonadotropic hormone PMSG in the inbred C57Bl/6J mice (viewed as a gold standard for diverse biomedical studies) as well as in the first generation hybrid C57Bl/6J×СВА/ lac and СВА/lac×C57Bl/6J mice at various ages.. The most effective donors of GV oocytes were СВА/lac×C57Bl/6J mice (F1 hybrids) yielding 25±2 oocyte/mouse.. In contrast, a significantly smaller number of GV oocytes can be isolated from the ovaries of female C57Bl/6J or C57Bl/6J×СВА/lac mice under the same conditions.

    Article Title: Hydrodynamic resistance of a yeast clog
    Article Snippet: To enhance the contrast of the clogs, Hoffman contrast modulation [44] is used with a Zeiss EC Plan Neofluar objective (20× magnification).

    Article Title: Zebrafish Connexin 79.8 (Gja8a): a lens connexin used as an electrical synapse in some neurons
    Article Snippet: Following fixation, cells were probed with streptavidin-Cy3 (Jackson) and photographed on a Zeiss fluorescence microscope (Axiovert 200 with 40x, 0.5 NA Hoffman Modulation Contrast objective) with a Hamamatsu C4742-95 digital camera using HCImage software (Hamamatsu, Sewickley, PA).

    Inverted Microscopy:

    Article Title: Regulation of trophectoderm morphogenesis by small GTPase RHOA through HIPPO signaling-dependent and -independent mechanisms in mouse preimplantation development.
    Article Snippet: The trophectoderm (TE) is the first tissue to differentiate during the preimplantation development of the mammalian embryo.. It forms the outer layer of the blastocyst and is responsible for generating the blastocoel, a fluid-filled cavity whose expansion is essential for successful hatching and implantation.. Here, we investigated the role of the small GTPase RHOA in the morphogenesis of the TE, particularly its relationship with HIPPO signaling, using mouse embryos as a model. Inhibition of RHOA resulted in the failure to form a blastocoel and significantly altered the expression of numerous genes.

    Article Title: RHOA activity in expanding blastocysts is essential to regulate HIPPO-YAP signaling and to maintain the trophectoderm-specific gene expression program in a ROCK/actin filament-independent manner
    Article Snippet: Bright-field images of embryos were captured, using an AxioCam MRm digital camera attached to an Axiovert 200 inverted microscope with Hoffman modulation contrast optics (Carl Zeiss, Thornwood, NY, USA). .. Bright-field images of embryos were captured, using an AxioCam MRm digital camera attached to an Axiovert 200 inverted microscope with Hoffman modulation contrast optics (Carl Zeiss, Thornwood, NY, USA). ..

    Saline:

    Article Title: Embryoid body test with morphological and molecular endpoints implicates potential developmental toxicity of trans -resveratrol
    Article Snippet: .. Morphometric analyses EBs were placed together in a dish filled with phosphate-buffered saline for photography, using an AxioCam MRm digital camera connected to an Axiovert 200 microscope with Hoffman modulation-contrast optics (Carl Zeiss, Thornwood, NY). ..



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    Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or <t>Hoffman</t> <t>Modulation</t> Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.
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    Image Search Results


    Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or Hoffman Modulation Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

    Journal: Cells

    Article Title: The LEDGF/p75 Integrase Binding Domain Interactome Contributes to the Survival, Clonogenicity, and Tumorsphere Formation of Docetaxel-Resistant Prostate Cancer Cells

    doi: 10.3390/cells10102723

    Figure Lengend Snippet: Knockdown of LEDGF/p75, JPO2, menin, or HRP2 impairs tumorsphere formation in PC3-DR and DU145-DR cells. siRNA-mediated knockdowns of LEDGF/p75 ( A ), JPO2 ( B ), menin ( C ), and HRP2 ( D ) were confirmed by immunoblotting in PC3-DR and DU145-DR cells transfected with specific siRNAs (KD) compared to cells transfected with SD control oligos. Representative images of tumorsphere formation assays showing a decrease in spheroid size in PC3-DR and DU145-DR cells after individual siRNA-mediated depletion of LEDGF/p75, JPO2, menin, or HRP2 compared to SD control spheres. Tumorspheres were visualized using phase contrast or Hoffman Modulation Contrast microscopy. Tumorsphere area was quantified (bottom graphs) from triplicate images per experiment using ImageJ software. Data are from at least 3 independent experiments and are represented as mean SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001.

    Article Snippet: Tumorspheres were grown for 4 days at 37 °C/5% CO 2 and visualized in an Olympus IX70 microscope equipped with Phase Contrast and Hoffman Modulation Contrast, and a SPOT imaging system.

    Techniques: Western Blot, Transfection, Microscopy, Software